| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
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| Autores: |
Morvezen, Romain
Cornette, Florence
Charrier, Gregory
Guinand, Bruno
Lapegue, Sylvie
Boudry, Pierre
Laroche, Jean
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| Data: |
2013-07
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| Ano: |
2013
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| Palavras-chave: |
Microsatellites
Polymerase chain reaction
Parentage
Assignment
Bivalve mollusc
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| Resumo: |
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00137/24822/22907.pdf
DOI:10.1051/alr/2013052
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| Editor: |
Edp Sciences S A
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| Relação: |
http://archimer.ifremer.fr/doc/00137/24822/
info:eu-repo/grantAgreement/EC/FP7/245119
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| Formato: |
application/pdf
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| Fonte: |
Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 207-213
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| Direitos: |
EDP Sciences, IFREMER, IRD 2013
info:eu-repo/semantics/openAccess
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